NeuroAdaptTrainer: A Fiji/ImageJ Plugin for YOLO-Based Neuron Segmentation, InteractiveCorrection and Transfer Learning
This work addresses the inefficiency and reliance on manual labor in neuronal segmentation and counting within neuroscience research by developing a Fiji/ImageJ plugin that integrates a YOLO-based instance segmentation model. The tool enables fully automated neuron detection, supports interactive manual correction, and incorporates online transfer learning using user-provided annotations to continuously refine the model. It also includes a built-in validation module for quantitative performance evaluation. By seamlessly embedding deep learning into established microscopy image analysis workflows, this approach significantly lowers the barrier for non-expert users to leverage state-of-the-art segmentation models while preserving expert oversight, thereby enhancing both segmentation efficiency and model adaptability.